Centrifuge Technique
5 Basic Principles of Centrifugation :
Particle Settling: During centrifugation, particles move and settle based on differences in their size, shape, and density.
Force Generation: Spinning creates a centrifugal force that pushes substances outward from the center, enabling separation.
Density-Based Movement: In density gradient centrifugation, components travel through a medium until they reach a layer matching their own density.
Separation Speed: Heavier and larger particles settle more quickly than lighter and smaller ones under the same conditions.
Rotor and Speed Impact: The outcome of centrifugation is influenced by the rotor’s design and how fast it spins.
